SARS-CoV-2 entry factors are highly expressed in nasal epithelial cells together with innate immune genes.
Identifieur interne : 000683 ( Main/Exploration ); précédent : 000682; suivant : 000684SARS-CoV-2 entry factors are highly expressed in nasal epithelial cells together with innate immune genes.
Auteurs : Waradon Sungnak [Royaume-Uni] ; Ni Huang [Royaume-Uni] ; Christophe Bécavin [France] ; Marijn Berg [Pays-Bas] ; Rachel Queen [Royaume-Uni] ; Monika Litvinukova [Royaume-Uni, Allemagne] ; Carlos Talavera-L Pez [Royaume-Uni] ; Henrike Maatz [Allemagne] ; Daniel Reichart [États-Unis] ; Fotios Sampaziotis [Royaume-Uni] ; Kaylee B. Worlock [Royaume-Uni] ; Masahiro Yoshida [Royaume-Uni] ; Josephine L. Barnes [Royaume-Uni]Source :
- Nature medicine [ 1546-170X ] ; 2020.
Abstract
We investigated SARS-CoV-2 potential tropism by surveying expression of viral entry-associated genes in single-cell RNA-sequencing data from multiple tissues from healthy human donors. We co-detected these transcripts in specific respiratory, corneal and intestinal epithelial cells, potentially explaining the high efficiency of SARS-CoV-2 transmission. These genes are co-expressed in nasal epithelial cells with genes involved in innate immunity, highlighting the cells' potential role in initial viral infection, spread and clearance. The study offers a useful resource for further lines of inquiry with valuable clinical samples from COVID-19 patients and we provide our data in a comprehensive, open and user-friendly fashion at www.covid19cellatlas.org.
DOI: 10.1038/s41591-020-0868-6
PubMed: 32327758
Affiliations:
- Allemagne, France, Pays-Bas, Royaume-Uni, États-Unis
- Angleterre, Angleterre de l'Est, Berlin, Grand Londres, Groningue (province), Massachusetts
- Berlin, Cambridge, Groningue, Londres
- University College de Londres, Université de Cambridge
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">SARS-CoV-2 entry factors are highly expressed in nasal epithelial cells together with innate immune genes.</title><author><name sortKey="Sungnak, Waradon" sort="Sungnak, Waradon" uniqKey="Sungnak W" first="Waradon" last="Sungnak">Waradon Sungnak</name><affiliation wicri:level="1"><nlm:affiliation>Wellcome Sanger Institute, Cambridge, UK. ws4@sanger.ac.uk.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Wellcome Sanger Institute, Cambridge</wicri:regionArea><wicri:noRegion>Cambridge</wicri:noRegion></affiliation></author><author><name sortKey="Huang, Ni" sort="Huang, Ni" uniqKey="Huang N" first="Ni" last="Huang">Ni Huang</name><affiliation wicri:level="1"><nlm:affiliation>Wellcome Sanger Institute, Cambridge, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Wellcome Sanger Institute, Cambridge</wicri:regionArea><wicri:noRegion>Cambridge</wicri:noRegion></affiliation></author><author><name sortKey="Becavin, Christophe" sort="Becavin, Christophe" uniqKey="Becavin C" first="Christophe" last="Bécavin">Christophe Bécavin</name><affiliation wicri:level="1"><nlm:affiliation>Université Côte d'Azur, CNRS, IPMC, Sophia-Antipolis, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Université Côte d'Azur, CNRS, IPMC, Sophia-Antipolis</wicri:regionArea><wicri:noRegion>Sophia-Antipolis</wicri:noRegion><wicri:noRegion>Sophia-Antipolis</wicri:noRegion></affiliation></author><author><name sortKey="Berg, Marijn" sort="Berg, Marijn" uniqKey="Berg M" first="Marijn" last="Berg">Marijn Berg</name><affiliation wicri:level="3"><nlm:affiliation>Department of Pathology and Medical Biology, University Medical Centre Groningen, University of Groningen, Groningen, the Netherlands.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Department of Pathology and Medical Biology, University Medical Centre Groningen, University of Groningen, Groningen</wicri:regionArea><placeName><settlement type="city">Groningue</settlement><region nuts="2" type="province">Groningue (province)</region></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Groningen Research Institute for Asthma and COPD, University Medical Centre Groningen, University of Groningen, Groningen, the Netherlands.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Groningen Research Institute for Asthma and COPD, University Medical Centre Groningen, University of Groningen, Groningen</wicri:regionArea><placeName><settlement type="city">Groningue</settlement><region nuts="2" type="province">Groningue (province)</region></placeName></affiliation></author><author><name sortKey="Queen, Rachel" sort="Queen, Rachel" uniqKey="Queen R" first="Rachel" last="Queen">Rachel Queen</name><affiliation wicri:level="1"><nlm:affiliation>Bioinformatics Core Facility, Newcastle University Biosciences Institute, Faculty of Medical Sciences, Newcastle University, Newcastle-upon-Tyne, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Bioinformatics Core Facility, Newcastle University Biosciences Institute, Faculty of Medical Sciences, Newcastle University, Newcastle-upon-Tyne</wicri:regionArea><wicri:noRegion>Newcastle-upon-Tyne</wicri:noRegion></affiliation></author><author><name sortKey="Litvinukova, Monika" sort="Litvinukova, Monika" uniqKey="Litvinukova M" first="Monika" last="Litvinukova">Monika Litvinukova</name><affiliation wicri:level="1"><nlm:affiliation>Wellcome Sanger Institute, Cambridge, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Wellcome Sanger Institute, Cambridge</wicri:regionArea><wicri:noRegion>Cambridge</wicri:noRegion></affiliation><affiliation wicri:level="3"><nlm:affiliation>Cardiovascular and Metabolic Sciences, Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Cardiovascular and Metabolic Sciences, Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author><author><name sortKey="Talavera L Pez, Carlos" sort="Talavera L Pez, Carlos" uniqKey="Talavera L Pez C" first="Carlos" last="Talavera-L Pez">Carlos Talavera-L Pez</name><affiliation wicri:level="1"><nlm:affiliation>Wellcome Sanger Institute, Cambridge, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Wellcome Sanger Institute, Cambridge</wicri:regionArea><wicri:noRegion>Cambridge</wicri:noRegion></affiliation></author><author><name sortKey="Maatz, Henrike" sort="Maatz, Henrike" uniqKey="Maatz H" first="Henrike" last="Maatz">Henrike Maatz</name><affiliation wicri:level="3"><nlm:affiliation>Cardiovascular and Metabolic Sciences, Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Cardiovascular and Metabolic Sciences, Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author><author><name sortKey="Reichart, Daniel" sort="Reichart, Daniel" uniqKey="Reichart D" first="Daniel" last="Reichart">Daniel Reichart</name><affiliation wicri:level="2"><nlm:affiliation>Department of Genetics, Harvard Medical School, Boston, MA, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Genetics, Harvard Medical School, Boston, MA</wicri:regionArea><placeName><region type="state">Massachusetts</region></placeName></affiliation></author><author><name sortKey="Sampaziotis, Fotios" sort="Sampaziotis, Fotios" uniqKey="Sampaziotis F" first="Fotios" last="Sampaziotis">Fotios Sampaziotis</name><affiliation wicri:level="4"><nlm:affiliation>Wellcome and MRC Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Wellcome and MRC Cambridge Stem Cell Institute, University of Cambridge, Cambridge</wicri:regionArea><orgName type="university">Université de Cambridge</orgName><placeName><settlement type="city">Cambridge</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Angleterre de l'Est</region></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>Department of Medicine, Addenbrookes Hospital, Cambridge, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Department of Medicine, Addenbrookes Hospital, Cambridge</wicri:regionArea><wicri:noRegion>Cambridge</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Cambridge Liver Unit, Cambridge University Hospitals, Cambridge, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Cambridge Liver Unit, Cambridge University Hospitals, Cambridge</wicri:regionArea><wicri:noRegion>Cambridge</wicri:noRegion></affiliation></author><author><name sortKey="Worlock, Kaylee B" sort="Worlock, Kaylee B" uniqKey="Worlock K" first="Kaylee B" last="Worlock">Kaylee B. Worlock</name><affiliation wicri:level="4"><nlm:affiliation>UCL Respiratory, Division of Medicine, University College London, London, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>UCL Respiratory, Division of Medicine, University College London, London</wicri:regionArea><placeName><settlement type="city">Londres</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Grand Londres</region></placeName><orgName type="university">University College de Londres</orgName></affiliation></author><author><name sortKey="Yoshida, Masahiro" sort="Yoshida, Masahiro" uniqKey="Yoshida M" first="Masahiro" last="Yoshida">Masahiro Yoshida</name><affiliation wicri:level="4"><nlm:affiliation>UCL Respiratory, Division of Medicine, University College London, London, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>UCL Respiratory, Division of Medicine, University College London, London</wicri:regionArea><placeName><settlement type="city">Londres</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Grand Londres</region></placeName><orgName type="university">University College de Londres</orgName></affiliation></author><author><name sortKey="Barnes, Josephine L" sort="Barnes, Josephine L" uniqKey="Barnes J" first="Josephine L" last="Barnes">Josephine L. Barnes</name><affiliation wicri:level="4"><nlm:affiliation>UCL Respiratory, Division of Medicine, University College London, London, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>UCL Respiratory, Division of Medicine, University College London, London</wicri:regionArea><placeName><settlement type="city">Londres</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Grand Londres</region></placeName><orgName type="university">University College de Londres</orgName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32327758</idno><idno type="pmid">32327758</idno><idno type="doi">10.1038/s41591-020-0868-6</idno><idno type="wicri:Area/Main/Corpus">001617</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001617</idno><idno type="wicri:Area/Main/Curation">001617</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">001617</idno><idno type="wicri:Area/Main/Exploration">001617</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">SARS-CoV-2 entry factors are highly expressed in nasal epithelial cells together with innate immune genes.</title><author><name sortKey="Sungnak, Waradon" sort="Sungnak, Waradon" uniqKey="Sungnak W" first="Waradon" last="Sungnak">Waradon Sungnak</name><affiliation wicri:level="1"><nlm:affiliation>Wellcome Sanger Institute, Cambridge, UK. ws4@sanger.ac.uk.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Wellcome Sanger Institute, Cambridge</wicri:regionArea><wicri:noRegion>Cambridge</wicri:noRegion></affiliation></author><author><name sortKey="Huang, Ni" sort="Huang, Ni" uniqKey="Huang N" first="Ni" last="Huang">Ni Huang</name><affiliation wicri:level="1"><nlm:affiliation>Wellcome Sanger Institute, Cambridge, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Wellcome Sanger Institute, Cambridge</wicri:regionArea><wicri:noRegion>Cambridge</wicri:noRegion></affiliation></author><author><name sortKey="Becavin, Christophe" sort="Becavin, Christophe" uniqKey="Becavin C" first="Christophe" last="Bécavin">Christophe Bécavin</name><affiliation wicri:level="1"><nlm:affiliation>Université Côte d'Azur, CNRS, IPMC, Sophia-Antipolis, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Université Côte d'Azur, CNRS, IPMC, Sophia-Antipolis</wicri:regionArea><wicri:noRegion>Sophia-Antipolis</wicri:noRegion><wicri:noRegion>Sophia-Antipolis</wicri:noRegion></affiliation></author><author><name sortKey="Berg, Marijn" sort="Berg, Marijn" uniqKey="Berg M" first="Marijn" last="Berg">Marijn Berg</name><affiliation wicri:level="3"><nlm:affiliation>Department of Pathology and Medical Biology, University Medical Centre Groningen, University of Groningen, Groningen, the Netherlands.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Department of Pathology and Medical Biology, University Medical Centre Groningen, University of Groningen, Groningen</wicri:regionArea><placeName><settlement type="city">Groningue</settlement><region nuts="2" type="province">Groningue (province)</region></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Groningen Research Institute for Asthma and COPD, University Medical Centre Groningen, University of Groningen, Groningen, the Netherlands.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Groningen Research Institute for Asthma and COPD, University Medical Centre Groningen, University of Groningen, Groningen</wicri:regionArea><placeName><settlement type="city">Groningue</settlement><region nuts="2" type="province">Groningue (province)</region></placeName></affiliation></author><author><name sortKey="Queen, Rachel" sort="Queen, Rachel" uniqKey="Queen R" first="Rachel" last="Queen">Rachel Queen</name><affiliation wicri:level="1"><nlm:affiliation>Bioinformatics Core Facility, Newcastle University Biosciences Institute, Faculty of Medical Sciences, Newcastle University, Newcastle-upon-Tyne, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Bioinformatics Core Facility, Newcastle University Biosciences Institute, Faculty of Medical Sciences, Newcastle University, Newcastle-upon-Tyne</wicri:regionArea><wicri:noRegion>Newcastle-upon-Tyne</wicri:noRegion></affiliation></author><author><name sortKey="Litvinukova, Monika" sort="Litvinukova, Monika" uniqKey="Litvinukova M" first="Monika" last="Litvinukova">Monika Litvinukova</name><affiliation wicri:level="1"><nlm:affiliation>Wellcome Sanger Institute, Cambridge, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Wellcome Sanger Institute, Cambridge</wicri:regionArea><wicri:noRegion>Cambridge</wicri:noRegion></affiliation><affiliation wicri:level="3"><nlm:affiliation>Cardiovascular and Metabolic Sciences, Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Cardiovascular and Metabolic Sciences, Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author><author><name sortKey="Talavera L Pez, Carlos" sort="Talavera L Pez, Carlos" uniqKey="Talavera L Pez C" first="Carlos" last="Talavera-L Pez">Carlos Talavera-L Pez</name><affiliation wicri:level="1"><nlm:affiliation>Wellcome Sanger Institute, Cambridge, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Wellcome Sanger Institute, Cambridge</wicri:regionArea><wicri:noRegion>Cambridge</wicri:noRegion></affiliation></author><author><name sortKey="Maatz, Henrike" sort="Maatz, Henrike" uniqKey="Maatz H" first="Henrike" last="Maatz">Henrike Maatz</name><affiliation wicri:level="3"><nlm:affiliation>Cardiovascular and Metabolic Sciences, Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Cardiovascular and Metabolic Sciences, Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author><author><name sortKey="Reichart, Daniel" sort="Reichart, Daniel" uniqKey="Reichart D" first="Daniel" last="Reichart">Daniel Reichart</name><affiliation wicri:level="2"><nlm:affiliation>Department of Genetics, Harvard Medical School, Boston, MA, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Genetics, Harvard Medical School, Boston, MA</wicri:regionArea><placeName><region type="state">Massachusetts</region></placeName></affiliation></author><author><name sortKey="Sampaziotis, Fotios" sort="Sampaziotis, Fotios" uniqKey="Sampaziotis F" first="Fotios" last="Sampaziotis">Fotios Sampaziotis</name><affiliation wicri:level="4"><nlm:affiliation>Wellcome and MRC Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Wellcome and MRC Cambridge Stem Cell Institute, University of Cambridge, Cambridge</wicri:regionArea><orgName type="university">Université de Cambridge</orgName><placeName><settlement type="city">Cambridge</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Angleterre de l'Est</region></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>Department of Medicine, Addenbrookes Hospital, Cambridge, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Department of Medicine, Addenbrookes Hospital, Cambridge</wicri:regionArea><wicri:noRegion>Cambridge</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Cambridge Liver Unit, Cambridge University Hospitals, Cambridge, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Cambridge Liver Unit, Cambridge University Hospitals, Cambridge</wicri:regionArea><wicri:noRegion>Cambridge</wicri:noRegion></affiliation></author><author><name sortKey="Worlock, Kaylee B" sort="Worlock, Kaylee B" uniqKey="Worlock K" first="Kaylee B" last="Worlock">Kaylee B. Worlock</name><affiliation wicri:level="4"><nlm:affiliation>UCL Respiratory, Division of Medicine, University College London, London, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>UCL Respiratory, Division of Medicine, University College London, London</wicri:regionArea><placeName><settlement type="city">Londres</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Grand Londres</region></placeName><orgName type="university">University College de Londres</orgName></affiliation></author><author><name sortKey="Yoshida, Masahiro" sort="Yoshida, Masahiro" uniqKey="Yoshida M" first="Masahiro" last="Yoshida">Masahiro Yoshida</name><affiliation wicri:level="4"><nlm:affiliation>UCL Respiratory, Division of Medicine, University College London, London, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>UCL Respiratory, Division of Medicine, University College London, London</wicri:regionArea><placeName><settlement type="city">Londres</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Grand Londres</region></placeName><orgName type="university">University College de Londres</orgName></affiliation></author><author><name sortKey="Barnes, Josephine L" sort="Barnes, Josephine L" uniqKey="Barnes J" first="Josephine L" last="Barnes">Josephine L. Barnes</name><affiliation wicri:level="4"><nlm:affiliation>UCL Respiratory, Division of Medicine, University College London, London, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>UCL Respiratory, Division of Medicine, University College London, London</wicri:regionArea><placeName><settlement type="city">Londres</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Grand Londres</region></placeName><orgName type="university">University College de Londres</orgName></affiliation></author></analytic><series><title level="j">Nature medicine</title><idno type="eISSN">1546-170X</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We investigated SARS-CoV-2 potential tropism by surveying expression of viral entry-associated genes in single-cell RNA-sequencing data from multiple tissues from healthy human donors. We co-detected these transcripts in specific respiratory, corneal and intestinal epithelial cells, potentially explaining the high efficiency of SARS-CoV-2 transmission. These genes are co-expressed in nasal epithelial cells with genes involved in innate immunity, highlighting the cells' potential role in initial viral infection, spread and clearance. The study offers a useful resource for further lines of inquiry with valuable clinical samples from COVID-19 patients and we provide our data in a comprehensive, open and user-friendly fashion at www.covid19cellatlas.org.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">32327758</PMID><DateRevised><Year>2020</Year><Month>08</Month><Day>12</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1546-170X</ISSN><JournalIssue CitedMedium="Internet"><Volume>26</Volume><Issue>5</Issue><PubDate><Year>2020</Year><Month>05</Month></PubDate></JournalIssue><Title>Nature medicine</Title><ISOAbbreviation>Nat. Med.</ISOAbbreviation></Journal><ArticleTitle>SARS-CoV-2 entry factors are highly expressed in nasal epithelial cells together with innate immune genes.</ArticleTitle><Pagination><MedlinePgn>681-687</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1038/s41591-020-0868-6</ELocationID><Abstract><AbstractText>We investigated SARS-CoV-2 potential tropism by surveying expression of viral entry-associated genes in single-cell RNA-sequencing data from multiple tissues from healthy human donors. We co-detected these transcripts in specific respiratory, corneal and intestinal epithelial cells, potentially explaining the high efficiency of SARS-CoV-2 transmission. These genes are co-expressed in nasal epithelial cells with genes involved in innate immunity, highlighting the cells' potential role in initial viral infection, spread and clearance. The study offers a useful resource for further lines of inquiry with valuable clinical samples from COVID-19 patients and we provide our data in a comprehensive, open and user-friendly fashion at www.covid19cellatlas.org.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Sungnak</LastName><ForeName>Waradon</ForeName><Initials>W</Initials><Identifier Source="ORCID">http://orcid.org/0000-0002-0136-4960</Identifier><AffiliationInfo><Affiliation>Wellcome Sanger Institute, Cambridge, UK. ws4@sanger.ac.uk.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Huang</LastName><ForeName>Ni</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Wellcome Sanger Institute, Cambridge, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bécavin</LastName><ForeName>Christophe</ForeName><Initials>C</Initials><Identifier Source="ORCID">http://orcid.org/0000-0003-1555-3153</Identifier><AffiliationInfo><Affiliation>Université Côte d'Azur, CNRS, IPMC, Sophia-Antipolis, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Berg</LastName><ForeName>Marijn</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Pathology and Medical Biology, University Medical Centre Groningen, University of Groningen, Groningen, the Netherlands.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Groningen Research Institute for Asthma and COPD, University Medical Centre Groningen, University of Groningen, Groningen, the Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Queen</LastName><ForeName>Rachel</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Bioinformatics Core Facility, Newcastle University Biosciences Institute, Faculty of Medical Sciences, Newcastle University, Newcastle-upon-Tyne, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Litvinukova</LastName><ForeName>Monika</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Wellcome Sanger Institute, Cambridge, UK.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Cardiovascular and Metabolic Sciences, Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Talavera-López</LastName><ForeName>Carlos</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Wellcome Sanger Institute, Cambridge, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Maatz</LastName><ForeName>Henrike</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Cardiovascular and Metabolic Sciences, Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Reichart</LastName><ForeName>Daniel</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Department of Genetics, Harvard Medical School, Boston, MA, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sampaziotis</LastName><ForeName>Fotios</ForeName><Initials>F</Initials><Identifier Source="ORCID">http://orcid.org/0000-0003-0812-7586</Identifier><AffiliationInfo><Affiliation>Wellcome and MRC Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Medicine, Addenbrookes Hospital, Cambridge, UK.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Cambridge Liver Unit, Cambridge University Hospitals, Cambridge, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Worlock</LastName><ForeName>Kaylee B</ForeName><Initials>KB</Initials><AffiliationInfo><Affiliation>UCL Respiratory, Division of Medicine, University College London, London, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yoshida</LastName><ForeName>Masahiro</ForeName><Initials>M</Initials><Identifier Source="ORCID">http://orcid.org/0000-0002-3521-5322</Identifier><AffiliationInfo><Affiliation>UCL Respiratory, Division of Medicine, University College London, London, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Barnes</LastName><ForeName>Josephine L</ForeName><Initials>JL</Initials><AffiliationInfo><Affiliation>UCL Respiratory, Division of Medicine, University College London, London, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><CollectiveName>HCA Lung Biological Network</CollectiveName></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>K08 HL130595</GrantID><Acronym>HL</Acronym><Agency>NHLBI NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>R01 HL145372</GrantID><Acronym>HL</Acronym><Agency>NHLBI NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>M899</GrantID><Agency>Rosetrees Trust</Agency><Country>International</Country></Grant><Grant><GrantID>PG/16/47/32156</GrantID><Acronym>BHF_</Acronym><Agency>British Heart Foundation</Agency><Country>United Kingdom</Country></Grant><Grant><GrantID>R01 HL146557</GrantID><Acronym>HL</Acronym><Agency>NHLBI NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>G2987</GrantID><Agency>Newton Fund</Agency><Country>International</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>04</Month><Day>23</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Nat Med</MedlineTA><NlmUniqueID>9502015</NlmUniqueID><ISSNLinking>1078-8956</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="CommentIn"><RefSource>J Allergy Clin Immunol Pract. 2020 Sep;8(8):2481-2483</RefSource><PMID Version="1">32485236</PMID></CommentsCorrections><CommentsCorrections RefType="CommentIn"><RefSource>Am J Otolaryngol. 2020 Sep - Oct;41(5):102548</RefSource><PMID Version="1">32516656</PMID></CommentsCorrections><CommentsCorrections RefType="UpdateOf"><RefSource>ArXiv. 2020 Mar 13;:</RefSource><PMID Version="1">32550242</PMID></CommentsCorrections></CommentsCorrectionsList><InvestigatorList><Investigator ValidYN="Y"><LastName>Banovich</LastName><ForeName>Nicholas E</ForeName><Initials>NE</Initials></Investigator><Investigator ValidYN="Y"><LastName>Barbry</LastName><ForeName>Pascal</ForeName><Initials>P</Initials></Investigator><Investigator ValidYN="Y"><LastName>Brazma</LastName><ForeName>Alvis</ForeName><Initials>A</Initials></Investigator><Investigator ValidYN="Y"><LastName>Collin</LastName><ForeName>Joseph</ForeName><Initials>J</Initials></Investigator><Investigator ValidYN="Y"><LastName>Desai</LastName><ForeName>Tushar J</ForeName><Initials>TJ</Initials></Investigator><Investigator ValidYN="Y"><LastName>Duong</LastName><ForeName>Thu Elizabeth</ForeName><Initials>TE</Initials></Investigator><Investigator ValidYN="Y"><LastName>Eickelberg</LastName><ForeName>Oliver</ForeName><Initials>O</Initials></Investigator><Investigator ValidYN="Y"><LastName>Falk</LastName><ForeName>Christine</ForeName><Initials>C</Initials></Investigator><Investigator ValidYN="Y"><LastName>Farzan</LastName><ForeName>Michael</ForeName><Initials>M</Initials></Investigator><Investigator ValidYN="Y"><LastName>Glass</LastName><ForeName>Ian</ForeName><Initials>I</Initials></Investigator><Investigator ValidYN="Y"><LastName>Gupta</LastName><ForeName>Ravindra K</ForeName><Initials>RK</Initials></Investigator><Investigator ValidYN="Y"><LastName>Haniffa</LastName><ForeName>Muzlifah</ForeName><Initials>M</Initials></Investigator><Investigator ValidYN="Y"><LastName>Horvath</LastName><ForeName>Peter</ForeName><Initials>P</Initials></Investigator><Investigator ValidYN="Y"><LastName>Hubner</LastName><ForeName>Norbert</ForeName><Initials>N</Initials></Investigator><Investigator ValidYN="Y"><LastName>Hung</LastName><ForeName>Deborah</ForeName><Initials>D</Initials></Investigator><Investigator ValidYN="Y"><LastName>Kaminski</LastName><ForeName>Naftali</ForeName><Initials>N</Initials></Investigator><Investigator ValidYN="Y"><LastName>Krasnow</LastName><ForeName>Mark</ForeName><Initials>M</Initials></Investigator><Investigator ValidYN="Y"><LastName>Kropski</LastName><ForeName>Jonathan A</ForeName><Initials>JA</Initials></Investigator><Investigator ValidYN="Y"><LastName>Kuhnemund</LastName><ForeName>Malte</ForeName><Initials>M</Initials></Investigator><Investigator ValidYN="Y"><LastName>Lako</LastName><ForeName>Majlinda</ForeName><Initials>M</Initials></Investigator><Investigator ValidYN="Y"><LastName>Lee</LastName><ForeName>Haeock</ForeName><Initials>H</Initials></Investigator><Investigator ValidYN="Y"><LastName>Leroy</LastName><ForeName>Sylvie</ForeName><Initials>S</Initials></Investigator><Investigator ValidYN="Y"><LastName>Linnarson</LastName><ForeName>Sten</ForeName><Initials>S</Initials></Investigator><Investigator ValidYN="Y"><LastName>Lundeberg</LastName><ForeName>Joakim</ForeName><Initials>J</Initials></Investigator><Investigator ValidYN="Y"><LastName>Meyer</LastName><ForeName>Kerstin B</ForeName><Initials>KB</Initials></Investigator><Investigator ValidYN="Y"><LastName>Miao</LastName><ForeName>Zhichao</ForeName><Initials>Z</Initials></Investigator><Investigator ValidYN="Y"><LastName>Misharin</LastName><ForeName>Alexander V</ForeName><Initials>AV</Initials></Investigator><Investigator ValidYN="Y"><LastName>Nawijn</LastName><ForeName>Martijn C</ForeName><Initials>MC</Initials></Investigator><Investigator ValidYN="Y"><LastName>Nikolic</LastName><ForeName>Marko Z</ForeName><Initials>MZ</Initials></Investigator><Investigator ValidYN="Y"><LastName>Noseda</LastName><ForeName>Michela</ForeName><Initials>M</Initials></Investigator><Investigator ValidYN="Y"><LastName>Ordovas-Montanes</LastName><ForeName>Jose</ForeName><Initials>J</Initials></Investigator><Investigator ValidYN="Y"><LastName>Oudit</LastName><ForeName>Gavin Y</ForeName><Initials>GY</Initials></Investigator><Investigator ValidYN="Y"><LastName>Pe'er</LastName><ForeName>Dana</ForeName><Initials>D</Initials></Investigator><Investigator ValidYN="Y"><LastName>Powell</LastName><ForeName>Joseph</ForeName><Initials>J</Initials></Investigator><Investigator ValidYN="Y"><LastName>Quake</LastName><ForeName>Steve</ForeName><Initials>S</Initials></Investigator><Investigator ValidYN="Y"><LastName>Rajagopal</LastName><ForeName>Jay</ForeName><Initials>J</Initials></Investigator><Investigator ValidYN="Y"><LastName>Tata</LastName><ForeName>Purushothama Rao</ForeName><Initials>PR</Initials></Investigator><Investigator ValidYN="Y"><LastName>Rawlins</LastName><ForeName>Emma L</ForeName><Initials>EL</Initials></Investigator><Investigator ValidYN="Y"><LastName>Regev</LastName><ForeName>Aviv</ForeName><Initials>A</Initials></Investigator><Investigator ValidYN="Y"><LastName>Reyfman</LastName><ForeName>Paul A</ForeName><Initials>PA</Initials></Investigator><Investigator ValidYN="Y"><LastName>Rozenblatt-Rosen</LastName><ForeName>Orit</ForeName><Initials>O</Initials></Investigator><Investigator ValidYN="Y"><LastName>Saeb-Parsy</LastName><ForeName>Kourosh</ForeName><Initials>K</Initials></Investigator><Investigator ValidYN="Y"><LastName>Samakovlis</LastName><ForeName>Christos</ForeName><Initials>C</Initials></Investigator><Investigator ValidYN="Y"><LastName>Schiller</LastName><ForeName>Herbert B</ForeName><Initials>HB</Initials></Investigator><Investigator ValidYN="Y"><LastName>Schultze</LastName><ForeName>Joachim L</ForeName><Initials>JL</Initials></Investigator><Investigator ValidYN="Y"><LastName>Seibold</LastName><ForeName>Max A</ForeName><Initials>MA</Initials></Investigator><Investigator ValidYN="Y"><LastName>Seidman</LastName><ForeName>Christine E</ForeName><Initials>CE</Initials></Investigator><Investigator ValidYN="Y"><LastName>Seidman</LastName><ForeName>Jonathan G</ForeName><Initials>JG</Initials></Investigator><Investigator ValidYN="Y"><LastName>Shalek</LastName><ForeName>Alex K</ForeName><Initials>AK</Initials></Investigator><Investigator ValidYN="Y"><LastName>Shepherd</LastName><ForeName>Douglas</ForeName><Initials>D</Initials></Investigator><Investigator ValidYN="Y"><LastName>Spence</LastName><ForeName>Jason</ForeName><Initials>J</Initials></Investigator><Investigator ValidYN="Y"><LastName>Spira</LastName><ForeName>Avi</ForeName><Initials>A</Initials></Investigator><Investigator ValidYN="Y"><LastName>Sun</LastName><ForeName>Xin</ForeName><Initials>X</Initials></Investigator><Investigator ValidYN="Y"><LastName>Teichmann</LastName><ForeName>Sarah A</ForeName><Initials>SA</Initials></Investigator><Investigator ValidYN="Y"><LastName>Theis</LastName><ForeName>Fabian J</ForeName><Initials>FJ</Initials></Investigator><Investigator ValidYN="Y"><LastName>Tsankov</LastName><ForeName>Alexander M</ForeName><Initials>AM</Initials></Investigator><Investigator ValidYN="Y"><LastName>Vallier</LastName><ForeName>Ludovic</ForeName><Initials>L</Initials></Investigator><Investigator ValidYN="Y"><LastName>van den Berge</LastName><ForeName>Maarten</ForeName><Initials>M</Initials></Investigator><Investigator ValidYN="Y"><LastName>Whitsett</LastName><ForeName>Jeffrey</ForeName><Initials>J</Initials></Investigator><Investigator ValidYN="Y"><LastName>Xavier</LastName><ForeName>Ramnik</ForeName><Initials>R</Initials></Investigator><Investigator ValidYN="Y"><LastName>Xu</LastName><ForeName>Yan</ForeName><Initials>Y</Initials></Investigator><Investigator ValidYN="Y"><LastName>Zaragosi</LastName><ForeName>Laure-Emmanuelle</ForeName><Initials>LE</Initials></Investigator><Investigator ValidYN="Y"><LastName>Zerti</LastName><ForeName>Darin</ForeName><Initials>D</Initials></Investigator><Investigator ValidYN="Y"><LastName>Zhang</LastName><ForeName>Hongbo</ForeName><Initials>H</Initials></Investigator><Investigator ValidYN="Y"><LastName>Zhang</LastName><ForeName>Kun</ForeName><Initials>K</Initials></Investigator><Investigator ValidYN="Y"><LastName>Rojas</LastName><ForeName>Mauricio</ForeName><Initials>M</Initials></Investigator><Investigator ValidYN="Y"><LastName>Figueiredo</LastName><ForeName>Francisco</ForeName><Initials>F</Initials></Investigator></InvestigatorList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>03</Month><Day>10</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>03</Month><Day>31</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>4</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>4</Month><Day>25</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>4</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32327758</ArticleId><ArticleId IdType="doi">10.1038/s41591-020-0868-6</ArticleId><ArticleId IdType="pii">10.1038/s41591-020-0868-6</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li><li>France</li><li>Pays-Bas</li><li>Royaume-Uni</li><li>États-Unis</li></country><region><li>Angleterre</li><li>Angleterre de l'Est</li><li>Berlin</li><li>Grand Londres</li><li>Groningue (province)</li><li>Massachusetts</li></region><settlement><li>Berlin</li><li>Cambridge</li><li>Groningue</li><li>Londres</li></settlement><orgName><li>University College de Londres</li><li>Université de Cambridge</li></orgName></list><tree><country name="Royaume-Uni"><noRegion><name sortKey="Sungnak, Waradon" sort="Sungnak, Waradon" uniqKey="Sungnak W" first="Waradon" last="Sungnak">Waradon Sungnak</name></noRegion><name sortKey="Barnes, Josephine L" sort="Barnes, Josephine L" uniqKey="Barnes J" first="Josephine L" last="Barnes">Josephine L. Barnes</name><name sortKey="Huang, Ni" sort="Huang, Ni" uniqKey="Huang N" first="Ni" last="Huang">Ni Huang</name><name sortKey="Litvinukova, Monika" sort="Litvinukova, Monika" uniqKey="Litvinukova M" first="Monika" last="Litvinukova">Monika Litvinukova</name><name sortKey="Queen, Rachel" sort="Queen, Rachel" uniqKey="Queen R" first="Rachel" last="Queen">Rachel Queen</name><name sortKey="Sampaziotis, Fotios" sort="Sampaziotis, Fotios" uniqKey="Sampaziotis F" first="Fotios" last="Sampaziotis">Fotios Sampaziotis</name><name sortKey="Sampaziotis, Fotios" sort="Sampaziotis, Fotios" uniqKey="Sampaziotis F" first="Fotios" last="Sampaziotis">Fotios Sampaziotis</name><name sortKey="Sampaziotis, Fotios" sort="Sampaziotis, Fotios" uniqKey="Sampaziotis F" first="Fotios" last="Sampaziotis">Fotios Sampaziotis</name><name sortKey="Talavera L Pez, Carlos" sort="Talavera L Pez, Carlos" uniqKey="Talavera L Pez C" first="Carlos" last="Talavera-L Pez">Carlos Talavera-L Pez</name><name sortKey="Worlock, Kaylee B" sort="Worlock, Kaylee B" uniqKey="Worlock K" first="Kaylee B" last="Worlock">Kaylee B. Worlock</name><name sortKey="Yoshida, Masahiro" sort="Yoshida, Masahiro" uniqKey="Yoshida M" first="Masahiro" last="Yoshida">Masahiro Yoshida</name></country><country name="France"><noRegion><name sortKey="Becavin, Christophe" sort="Becavin, Christophe" uniqKey="Becavin C" first="Christophe" last="Bécavin">Christophe Bécavin</name></noRegion></country><country name="Pays-Bas"><region name="Groningue (province)"><name sortKey="Berg, Marijn" sort="Berg, Marijn" uniqKey="Berg M" first="Marijn" last="Berg">Marijn Berg</name></region><name sortKey="Berg, Marijn" sort="Berg, Marijn" uniqKey="Berg M" first="Marijn" last="Berg">Marijn Berg</name></country><country name="Allemagne"><region name="Berlin"><name sortKey="Litvinukova, Monika" sort="Litvinukova, Monika" uniqKey="Litvinukova M" first="Monika" last="Litvinukova">Monika Litvinukova</name></region><name sortKey="Maatz, Henrike" sort="Maatz, Henrike" uniqKey="Maatz H" first="Henrike" last="Maatz">Henrike Maatz</name></country><country name="États-Unis"><region name="Massachusetts"><name sortKey="Reichart, Daniel" sort="Reichart, Daniel" uniqKey="Reichart D" first="Daniel" last="Reichart">Daniel Reichart</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/CovidFranceV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000683 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000683 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= CovidFranceV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:32327758
|texte= SARS-CoV-2 entry factors are highly expressed in nasal epithelial cells together with innate immune genes.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:32327758" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a CovidFranceV1
| This area was generated with Dilib version V0.6.37. |  |